Method and Materials


Ø  METHOD

1.     Take the materials we are going to use.
2.     Weight 5,00g g of tobacco in a beaker
3.     Mash the sample with the mortar.
4.     Add 150 mL of distil water and shake it with a stirring rod during 15 minutes.
5.     When no more of the solute is able to be dissolved, put the mixture into the magnetic stirrer.
6.     When the solution is finished, prepare 6 bacteria cultures in 6 Erlenmeyer flasks. Using this method:
a)     We took 2mm of the solution and we put it in each of the cuvettes, we need 7 of them.
b)     Knowing that each of the solutions have different quantities of bacteria, we introduced each of them in the spectrometer to see the amount of bacteria each of them have.
c)     We do these steps with the 7 different solutions we have.

7.     Compare the effect produced in the six cultures by the cigarette solution and write down any relevant data or information.

Ø  MATERIALS 

o   Petri dish
o   Bacteria
o   Cigarettes
o   Mortar
o   Magnetic stirrer
o   Spectrometer
o   Beaker
o   Aluminium foil
o   Erlenmeyer flasks

o   Pot

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