Conclusion

At first, we thought that the solution of tobacco was going to be detrimental towards life. As many people know, a lot of the substances found in tobacco are harmful for the organism, so our first hypothesis was that the more tobacco we added to the cultures the least amount of bacteria that would be grown. We thought that the conditions formed by the tobacco solution would difficult the growth of bacteria, as some of the substances in tobacco seriously harm the cells in our body.

Looking at our results we can infer some general conclusions. In or first culture we only added 30mL of water as a base culture, without adding any tobacco. We did this for comparing some base results with tobacco results and notice some differences with the addition of tobacco. At wavelength 602, 8 absorbance of light was 0, 90473.

On the second culture, in which we added a tobacco solution with a 0, 0033 gr/mL concentration, the absorbance of light at standardize wavelength was 0, 90016. As we can see the absorbance decreases from the base culture to the 0, 0033 gr/mL concentration cultures.  This could be caused by an anomaly because, from now on, the absorbance increases linearly.

The increase of absorbance of light means an increase in the amount of bacteria found in the culture. The reason for this is that if there are a lot of bacteria in the culture it would become more turbid. This turbidity would make less light pass through the liquid and more of it would be absorbed.

These results clearly contradict our first hypothesis. At first we thought that if we introduce a higher amount of tobacco, its substances would have killed the bacteria but in fact, it stimulated the bacterial growth.

A study, published by Maryland University investigators, in the magazine Environmental Health Perspectives, affirms that some bacteria are inhaled alive while smoking and can be installed in the lungs. The scientist studied all the bacteria found on a cigarette and they found at least 700 different species of bacteria, many of them known to cause diseases. (Laborda, 2010)

Apart from the bacteria inhaled with tobacco our lungs contain other bacteria. According to our results the substances found in tobacco could favour the growth of pathogenic organisms with tobacco. Therefore we have seen that many of our results in tobacco worsen many conditions caused by tobacco initially. Apart from stimulating the carcinogenic growth of lung cells tobacco can also produce many diseases caused by bacteria.

This clearly shows how harmful is tobacco for our organism and how little are we aware of what we are smoking and of the many consequences that smoking could have in the long and short term for us.